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Back to category: Science Limited version - please login or register to view the entire paper. Thin Layer Chromatography Purpose: The purpose of this experiment is to identify the contents of four samples, therefore determining the purity with the use of thin-layer chromatography. Theory: Chromatography is a separation method that relies on differences in partitioning behavior between a moving phase and a stationary phase to separate the components in a mixture. In thin-layer chromatography, the moving phase is a solvent and the stationary phase is a solid adsorbent on a plate (sheet of glass, metal, or plastic) coated with a thin layer of a solid adsorbent. The solvent used in today’s experiment is chloroform which is a polar liquid and the solid adsorbent is a silica gel which attracts polar molecules. A small amount of the mixture to be analyzed is spotted near the bottom of this plate. The plate is then placed in a shallow pool of the chloroform in a developing chamber so that only the very bottom of the plate is in the liquid. The chlor... Posted by: Jennifer Valles Limited version - please login or register to view the entire paper. |
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