Back to category: Science

Limited version - please login or register to view the entire paper.

The Principles and Applications of Thin Layer Chromatography (TLC)

Title : The Principles and Applications of Thin Layer Chromatography (TLC)
Name:
ID # :
Date : 15 September 2003
Aim : To examine and report the principles and applications of TLC


Thin Layer Chromatography (TLC) allows the separation and subsequent identification of components from one mixture using a thin layer of a chromatographic adsorbent on an inert rigid support. It was developed between the late thirties and the early to mid forties and is used commonly for the analysis of natural products. The separation depends on the choice of stationary and liquid phase.
The adsorbent material is usually solid and is most commonly, Silica gel. Other adsorbents include Alumina, Cellulose powders, Molecular sieve layers, Kieselguhr, Celite, Calcium Sulphate and Polyamide (Polyprolactam).
Solvents are usually organic with relatively low polarity. This is to minimise adsorption of any components of the solvent mixture. The purity of the solvent is extremely important due to t...

Posted by: Carmen hershman

Limited version - please login or register to view the entire paper.