Protein Determination in Powdered Milk (and Understanding of the Use andApplication of the Spectrophotometer)

Abstract:

Understanding the use of the spectrophotometer is useful when
conducting lab experiments where analysis of a solution is required to give
a numerical value for that solution. In situations where the
spectrophotometer is used, the Beer-Lambart equation can be used to find
the concentration of a solution when the absorbance of the solution is
known (which can be extrapolated through the use of the spectrophotometer).
The lab involves the use of a spectrophotometer to analyze the
concentration of protein in a sample of diluted milk reacted with a protein
assay reagent.


Introduction:

The general subject we are studying is protein determination in
powdered milk.

We will be using a spectrophotometer, which is an instrument that uses
light energy to determine the concentration of a solute by the solutes
absorption rate. Using standardized protein concentrations and the
spectrophotometer, we will be able to determine the protein concentration
in diluted mil...