MICRO

Introduction:

Microorganisms are extremely small and can be isolated by different methods. The purpose of this lab was to take an unknown mixture containing two different types of bacteria and isolate a pure culture of each type and be able to identify what each bacteria was. This was done using the procedures we have learned thus far in lab.
More than one type of organism may show up in the selective environments that were used, that is why we kept isolating the bacteria from day to day. A pure culture is not present until there is one type of colony. The unknown bacteria was identified by streaking them on different petri plates, such as; DNAse, Pseudomonas Afar P (PAP), Pseudomonas Agar F (PAF), Nutrient Agar (NA), Blood Agar Plate (BAP), Colistin-Nalidxic Agar (CNA), MacConkey Agar (MAC) and Trypticase Soy Broth (TSB) plates. TTC deeps, TSI slants, tryptone broth, bile esculin broth, gram stains, catalase and oxidase tests were also used.
The first day t...